spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 4


Fig. 4. MAPK activation and protein synthesis are required for early CPEB phosphorylation. (A) U0126 blocks CPEB phosphorylation. Oocytes were cultured in the presence of U0126, cycloheximide (cyclohex), or no additive (control) for 1 hour prior to the addition of progesterone (pg). Extracts were prepared from oocytes collected at the indicated times (hr in pg) and used in His-CPEB phosphorylation assays. Samples were analyzed by immunoblotting with anti-P-MAPK and MAPK antibodies (lower two panels). (B) Inactivation of MAPK reduces CPEB phosphorylation. Protein extracts from oocytes collected at the indicated times (hr in pg) were treated first with either Mkp1 or buffer, and then His-CPEB and [{gamma}-32P]ATP were added. Samples were analyzed as in A. Asterisk indicates 100% GVBD oocytes. (C) The extracts used in B were used in an H1 kinase assay. Asterisk indicates 100% GVBD oocytes. CS, Coomassie staining; 32P, autoradiography; IB, immunoblotting.





Right arrow Return to article