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Figure 4


Fig. 4. Perturbation of repair replication in NER enhances H2AX phosphorylation. (A,B) MSU-2 or XP2BI cells under growtharrested conditions were exposed to 10 J/m2 UV globally (A) or locally (B), incubated for 1 hour in the absence or presence of 100 µM Ara-C before fixation and stained with anti-{gamma}-H2AX antibody. (C) Growth-arrested MSU-2 cells were exposed to 10 J/m2 UV or 15 µg/mL etoposide and incubated for 1 hour in the presence (red lines) or absence (black lines) of Ara-C before fixation. Cells were stained with anti-{gamma}-H2AX antibody and PI and analyzed by flow cytometry.





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