spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 2


Fig. 2. Overexpression of MIIA tail-GFP prevents the ligand-induced endocytosis of CXCR4 in T cells. (A) Flow cytometry analysis of CXCR4 surface expression in untransfected J77 cells and J77 cells transfected with GFP, MIIA tail-GFP and MIIA head-GFP after 30-minute treatment with 300 nM SDF-1{alpha}, or 1-hour treatment PMA 100 ng/ml. CXCR4 plasma membrane expression levels in untreated samples were not altered by the transfection of the different GFP constructs and were in the range of 100-130 MFI units in different experiments. Data are represented as the mean ± s.d. of the percentages of CXCR4 surface expression of three independent experiments *P<0.05 or a representative experiment for MIIA head-GFP. (B) Flow cytometry analysis of cell surface expression of transferrin receptor after treatment with 500 µg/ml transferrin for 1 hour. Data represented are the mean ± s.d. of three independent experiments. (C) Flow cytometry analysis showing the kinetics of CXCR4 membrane expression in J77 cells untransfected or transfected with MIIA tail-GFP. Data represent the mean percentage of CXCR4 expression ± s.d. with respect to the expression at 0 minutes of three independent experiments.





Right arrow Return to article