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Fig. 2. Overexpression of DDAHI and DDAHII prevents ADMA-induced increase in stress fibre and focal adhesion formation and restores normal motility in ADMA-treated cells. (A-E) Cells incubated with ADMA and infected with control adenoviruses to express AdGFP. (F-O) Cells treated with ADMA and overexpressed AdDDAHI (F-J) and AdDDAHII (K-O). (P-T) Cells overexpressing AdDDAHI without the addition of ADMA. In A-T the columns from the left to right show F-actin distribution, vinculin distribution, the cells overexpressing recombinant proteins, composite images and cell trajectories, as indicated. In the composite images F-actin is red, vinculin is green and the cells overexpressing GFP are blue (pseudocolour). Bar, 10 µm (180 µm in E, J, O, T). (U) Changes in the cell speed; (W) changes in total cell translocation in non-treated cells and cells overexpressing AdGFP, AdDDAHI, AdDDAHII. The cells were left untreated or were treated with 100 µM of ADMA or SDMA, as indicated. *P<0.05; **P<0.01, comparison with untreated controls. (Bottom left) The two images are an example of fluorescently labelled cells expressing recombinant DDAHII that were used for cell tracking. Only overexpressing cells (96% of total cell population) were tracked.
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