QUICK SEARCH:

[advanced]

	Author:		Keyword(s):

Home Help Feedback Subscriptions Archive Search Table of Contents

	Help viewing high resolution images
	Return to article

(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.

Fig. 5. Overview of AIS localization and current levels for the various KCNQ constructs. (A) Summary of observed KCNQ AIS localization in neurons transfected with the listed combinations of constructs. AIS/distal axon (AIS/DA) and AIS/dendrite (AIS/den) ratios were determined for each combination of constructs for 29-30 cells in at least three different cultures. Q2+Q3 ${Delta}$ was statistically different from wild-type Q2+Q3 for both KCNQ2 and KCNQ3 immunofluorescence quantifications, whereas Q2 ${Delta}$ +Q3 was not. In addition, Q2+Q3 ${Delta}$ was statistically different from Q2 ${Delta}$ +Q3 ${Delta}$ in the KCNQ2 quantification, but not for the KCNQ3 quantification. One-way ANOVA and Bonferroni's post-test were used in the comparison of Q2+Q3 vs Q2 ${Delta}$ +Q3, Q2+Q3 vs Q2+Q3 ${Delta}$ and Q2 ${Delta}$ +Q3 vs Q2+Q3 ${Delta}$ (^*P<0.05, ^**P<0.001). (B) Summary of current density levels observed in neurons transfected with the listed constructs. All neurons were co-transfected with GFP. Recordings were carried out on cultures from at least two different days. Q2+Q3 and ${Delta}$ Q2+ ${Delta}$ Q3 were statistically different from controls. One-way ANOVA and Dunnet's multiple comparison post-test were used to compare the various constructs with the control (^*P<0.05). (A+B) Data are displayed as mean±standard error of mean. Abbreviations: control, GFP; Q2, hKCNQ2-cmyc; Q3, hKCNQ3-FLAG; Q2 ${Delta}$ , hKCNQ2-delAnkG; Q3 ${Delta}$ , hKCNQ3-delAnkG; Q2A, Q2-AAA; and Q3-A, Q3-AAA.

Return to article