spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 5


Fig. 5. BMP-9 activates the Smad1- and Smad5-responsive BRE luciferase reporter through ALK1. (A-B) BAECs were transiently transfected with an ID1-promoter-derived luciferase reporter construct (BRE) and a construct for beta-galactosidase as internal expression control. Before stimulation, cells were serum-starved for 8 hours and then incubated with the respective ligands overnight. (A) BRE activity is induced by BMP-9 (100 ng/ml) and BMP-6 (100 ng/ml) but not by TGF-beta (5 ng/ml). (B) BMP-9 (5 ng/ml) stimulated BRE-induction can be inhibited by a 15-fold molar excess of ALK1-Fc. (C,D) shRNA constructs against ALK1 or ALK2 were cloned into the pSuper vector and co-transfected into BAECs. ALK1 shRNA blocks BMP-9-stimulated (5 ng/ml) (C), but not BMP-6-induced (25 ng/ml) BRE activation (D). (E) ALK1 shRNA-mediated reduction of BRE activity is rescued by overexpression of human ALK1, but not by human ALK2. *P<0.01 compared with BMP-9-stimulated control; #P<0.01 compared with ALK1 RNAi after BMP-9 stimulation.





Right arrow Return to article