spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 3


Fig. 3. SSPN-Tg mice do not exhibit sarcolemma instability. (A) Evans Blue tracer assay for infiltration of blood serum proteins into the muscle fiber. Intraperitoneal injection of Evans Blue dye can be detected in muscle fibers with damaged sarcolemma. Transverse cross sections of quadriceps muscle were imaged using a fluorescence microscope equipped with green activation filters. An image from mdx muscle, which has severe membrane damage, is shown as a positive control for Evans Blue staining. Evans Blue dye was not detected in phenotypic or non-phenotypic (data not shown) SSPN-Tg mice. Bar, 50 µm. (B) Analysis of muscle-specific creatine kinase in circulating blood serum. Creatine kinase activities were evaluated in serum samples from non-Tg, mdx, and phenotypic SSPN-Tg mice. SSPN-Tg mice do not exhibit leakage of muscle-specific creatine kinase into the blood serum. Values are means and s.e.m. of total mice analyzed. *P=0.002 compared to control.





Right arrow Return to article