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Figure 4


Fig. 4. DGC proteins are increased upon SSPN overexpression. Immunoblot analysis of total proteins extracted from skeletal muscle of non-Tg (lane 1), non-phenotypic SSPN-Tg line 29.1 (lane 2), and phenotypic SSPN-Tg line 37.5 (lane 3) mice. Skeletal muscle was solubilized using 3% SDS buffer and protein samples (60 µg) were separated by SDS-PAGE and transferred to nitrocellulose membranes. Equal loading of protein samples was confirmed by Coomassie Blue (CB) stain. Immunoblots were probed with antibodies to laminin (Lam), dystrophin (Dys), {alpha}- and beta-DG, {alpha}-, beta-, and {gamma}-SG, exogenous SSPN (hSSPN), endogenous SSPN (mSSPN) and caveolin-3 (cav-3), as indicated.





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