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Figure 3


Fig. 3. siRNA-mediated knockdown of PKC{delta} in MDCK cells suppresses their migration. (A) Doxycycline-inducible expression of the PKC{delta} siRNA was established in MDCK cells. Parental MDCK cells, a control siRNA clone and two PKC{delta}-specific siRNA clones were grown in the medium supplemented with (+) or without (-) 2 µg/ml doxycycline for 72 hours before lysis. The expression and activity of PKC{delta} was analyzed. The expression levels of PKC{alpha}, PKC{epsilon} and tubulin were analyzed and served as the control. (B) Cells were grown in medium with (+) or without (-) 2 µg/ml doxycycline for 72 hours and then subjected to a Trans-well cell migration assay. Values (means ± s.d.) are from three independent experiments, relative to the level of the control MDCK cells, which was defined as 100%. *P<0.05 compared with their counterparts in the medium without doxycycline.





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