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Figure 5


Fig. 5. PKC{alpha} does not phosphorylate the Ser726 of {alpha}-adducin. (A) GFP, GFP-PKC{alpha} and GFP-PKC{delta} were transiently overexpressed in COS cells. Whole cell lysates were analyzed by immunoblotting with anti-phospho-adducin (Ser726), anti-adducin or anti-GFP. The Ser726 phosphorylation of adducin was measured and expressed as fold increase relative to the level in control GFP cells. (B) Endogenous PKC{alpha} was immunoprecipitated by anti-PKC{alpha}. The washed immunocomplexes were subjected to in vitro kinase assays using purified GFP-{alpha}-adducin (GFP-add) or its mutants as the substrate. The phosphorylation of GFP-{alpha}-adducin (wt) is defined as 100%. Values (means ± s.d.) are from three independent experiments. IVK, in vitro kinase assay.





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