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Figure 1


Fig. 1. Synapsin IIb mRNA and protein are expressed in adipocytes. (A) Northern blots of RNA samples (10 µg) from mouse brain (Br) or 3T3-L1 adipocytes (Ad) probed with a 185 bp pan-synapsin C domain probe, a 649 bp synapsin II C domain probe or a probe from the synapsin IIb 3'-untranslated region (UTR). (B,C) Anti-synapsin western blotting with two affinity-purified antibodies raised against the C domain of synapsin IIb (antibodies 69 and 83) from samples (10 µg) of (B) mouse brain post-nuclear supernatant or (C) 3T3-L1 adipocyte (L1), primary adipocyte (1°), or liver low density microsomes. Solid boxes indicate that the samples were run on the same gel; dashed lines indicate that the order of samples on the gel was altered in the image used in the figure.





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