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Figure 4


Fig. 4. S10A synapsin redistributes Glut4 in adipocytes. L1 adipocytes prepared as described in Fig. 3 were treated with or without insulin (100 nM, 30 minutes), then fixed and analyzed. zstack confocal images of GFP fluorescence were collected from ~60 infected adipocytes of each type and maximum projections were analyzed. (A) Representative images. (B) Percentage of cells in the maximum projections with Glut4 at or near the plasma membrane (rim + diffuse fluorescence) in basal (white) or insulin-stimulated (black) cells. (C) Average object sizes (contiguous area with green intensity greater than the threshold in maximum projections; mean ± s.e.m., standardized to Glut4 basal). (D) Histograms of intensity distributions (shown on z-axis) of GFP fluorescence in the representative images shown in A (G4, G4/S, G4/S10A, left to right).





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