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Figure 7


Fig. 7. Synapsin is phosphorylated at site 1 in basal adipocytes. Western blotting with site-1-specific phospho-synapsin (pSyn) and pansynapsin (Syn) antibodies. (A) Samples from mouse brain (Br) or 3T3-L1 adipocytes expressing WT-Flag-synapsin IIb (L1). (B) Samples from basal or insulin-stimulated (100 nM, 30 minutes) 3T3-L1 adipocytes expressing WT or S10A Flag-synapsin IIb. (C) Anti-Flag immunoprecipitates of WTFlag-synapsin incubated ± {lambda}-phosphatase ({lambda}-PPase) ± phosphatase inhibitors.





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