spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 4


Fig. 4. Endofin modulates Smad1 phosphorylation and nuclear translocation. (A) Silencing of endofin expression by RNA interference inhibited Smad1 phosphorylation. C2C12 cells were transfected with Flag-tagged Smad1 and either vector, construct for GFP or endofin siRNA. On the third day following 3-hour BMP2 treatment, Flag-tagged Smad1 was precipitated with anti-flag and its phosphorylation level was detected with anti-phospho-Smad1. (B) Endofin, but not SARA, regulates BMP-specific phosphorylation and intracellular translocation of Smad1. C2C12 cells were transfected with either HA-SARA or HA-Endofin, then stimulated with BMP (100 ng/ml) for 3 hours. After fixation with paraformaldehyde (PFA), cells were immunostained with either anti-HA (green) or anti-phospho-Smad1 (red). Nuclei were visualized using Hoechst 33342. Representative images are shown. Bar, 20 µm.





Right arrow Return to article