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Figure 2


Fig. 2. Microtubule targeting events correlate with filopodia turning and merging. B16F1 cells were co-transfected with YFP-fascin to mark filopodia and YFP-beta-tubulin to highlight microtubules. (A) The time-lapse series shows two microtubules (MT1 and MT2) targeting a filopodium (FL1) in a lamellipodium wing. The targeted filopodium (FL1) turns toward the lamellipodium midline. A nearby nontargeted filopodium (FL2) serves as a reference. (B) The targeted filopodium (FL1) turns and completely merges with a neighboring filopodium (FL2) at 50 seconds (asterisk). A nearby nontargeted filopodium (FL3) remains about its original position. (C) Time course of turning expressed as angle change in degrees of filopodia FL1 and FL2 shown in A. (D) Time course of turning and merging of the filopodia shown in B. A positive {Delta}{theta} value denotes filopodia turning towards the lamellipodium midline. (E) Time course of average difference in angle between targeted and reference filopodia pairs after the targeting event begins (n=15 targeting events). (F) Time course of cumulative percentage of the targeted filopodia that merge with neighboring filopodia after the targeting event begins (n=15 targeting events). Bars, 2 µm. Error bars indicate standard error.





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