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Figure 3


Fig. 3. Nocodazole uncouples targeting, decreases filopodia merging and increases filopodia in lamellipodia wings. B16F1 cells were allowed to spread on laminin for 30 minutes and then treated with 3 µM nocodazole for the indicated times. Cell were fixed and stained with anti-tubulin antibodies and phalloidin. (A) Time course of number of peripheral microtubules in cells treated with nocodazole. The number of peripheral microtubules rapidly decreases within 0.5 minutes. (B) Number of filopodia merging events in each lamellipodium zone in cells treated with nocodazole for 0, 0.5, 1.0, or 2.0 minutes. Merging is decreased in zones 3 at 1.0 and 2.0 minutes (compared with 0 minutes; *P<0.01, unpaired t-test, n=25 cells). (C) Number of filopodia in each zone in cells treated with nocodazole. Filopodia are increased in zones 3 at 1.0 and 2.0 minutes (compared with 0 minutes; *P<0.01, unpaired t-test, n=25 cells). Error bars indicate standard error.





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