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Figure 4


Fig. 4. Maintenance of condensin I is dependent on Aurora B activity. (A) Nocodazole-arrested HeLa cells either expressing EGFP-kleisin-{gamma} (condensin I) or EGFP-kleisin-beta (condensin II). At time point 0 (t=0), 100 nM Hesperadin was added to the cells and the levels of condensin followed over time. Hoechst 33342 (0.2 µg/ml) was used to stain chromatin. (B) EGFP-kleisin-{gamma} (condensin I) (red, n=25) and EGFP-kleisin-beta (condensin II) (blue, n=19) signals were quantified as in Fig. 1B and Fig. 1D over time. Note that the increase in EGFP-kleisin-beta (condensin II) visible in A upon Hesperadin treatment is masked by the normalisation with Hoechst, which likewise increases. Movies were taken on an Olympus DeltaVision microscope [40x objective, five slices every 2 minutes, 1x1 binning (EGFP-kleisin-{gamma}) and 2x2 binning (EGFP-kleisin-beta)]. Projected images are shown.





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