spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 4


Fig. 4. Effect of the overexpression of ALK5, ALK1 and Smad isoforms on ALK5- and ALK1-specific reporters and on human ET-1 promoter. The effect of the overexpression of constitutively active (ca) forms of ALK5 and ALK1 on (A) ALK5- and (B) ALK1-specific luciferase reporters was analyzed by cotransfection experiments in BAEC. Overexpression plasmids were cotransfected together with a -650/+172-bp fragment of the human ET-1 promoter linked to a luciferase gene. (C) Effect of ALK5 and ALK1 ca in the absence of TGF-beta (open bars), and of ALK5 and ALK1 wild type (wt) and kinase-deficient (kd) forms in the presence of 5 ng/ml TGF-beta (filled bars). (D) Effect of Smad3, Smad1 and Smad5 overexpression in the presence of TGF-beta. Luciferase activity was measured by luminometry and expressed as fold induction with respect to control pCMV5 empty vector (mean ± s.d., n=4, *P<0.05 versus control).





Right arrow Return to article