|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
|
Fig. 10. SW13-cl2 cells stably transfected with GFAP and transiently cotransfected with vimentin and 
-synemin. Double immunofluorescence staining for vimentin (A) and synemin (B) was performed 48 hours after transfection. Confocal microscopy revealed that synemin associated with the IF network in cells transfected with vimentin. (C,F) SW13-cl2 cells were stably transfected with vimentin and transiently cotransfected with GFAP and 
-synemin, and double immunofluorescence staining with anti-GFAP (C,E) and anti-synemin (D,F) was performed 48 hours after transfection. In vimentin-containing cells transiently cotransfected with synemin and GFAP, confocal microscopy showed that synemin incorporated into the entire GFAP network (D,F). (E,F) Synemin also incorporated into the most peripheral area of the GFAP network, where it is the least dense. Bars, 10 µm (A-D); 1 µm (E,F).
|
