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Fig. 2. Synemin expression levels in the cerebral hemisphere of 2-day-old wild-type (wt) and Gfap^-^/^-Vim^-^/^- (G-/V-) mice. (A) Western blots show that synemin protein levels are markedly lower in G-/V-than wild-type mice (upper band, ${alpha}$ -synemin; lower band, $beta$ -synemin) and confirm the absence of GFAP and vimentin in G-/V-mice. Actin control blots demonstrate equal protein loading. (B) A 0.8% ethidium-bromide agarose gel loaded with RT-PCR products obtained after amplification with synemin primers shows two bands corresponding to ${alpha}$ - and $beta$ -synemin mRNA in wild-type and G-/V-mice. Lane M shows molecular mass markers, numbers indicate kb. (C) TaqMan real-time PCR amplification curves show no difference in synemin mRNA levels between wild-type mice (circles) and G-/V-mice (triangles). ${Delta}$ Rn, log fluorescence emission intensity of the reporter dye normalized to that of a passive reference dye. Values are mean ± s.e.m. of four different samples. Horizontal line indicates background fluorescence level.

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