spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 6


Fig. 6. (A) Coomassie Blue-stained 4-15% SDS-polyacrylamide gel shows bacterially expressed {alpha}-synemin (lane 1), beta-synemin (lane 2), GFAP (lane 3) and vimentin (lane 4) after purification through polymerization/depolymerization cycles and gel filtration; numbers to the left indicate kDa. (B) Overlay assays to assess binding of {alpha}-synemin to vimentin, GFAP and actin. Upper panel: Coomassie Blue-stained gel demonstrates equal protein loading. Middle panel: western blot incubated with {alpha}-synemin and then with anti-synemin followed by a secondary antibody. {alpha}-synemin bound equally well to monomeric GFAP and vimentin but did not bind to actin. Lower panel: similar western blot incubated with anti-synemin followed by a secondary antibody. (C) Dot-blot assay in which decreasing amounts of GFAP, vimentin and actin were adsorbed to nitrocellulose under nondenaturing, nonpolymerizing conditions. Synemin bound similarly to GFAP and vimentin but did not bind to actin.





Right arrow Return to article