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Figure 3


Fig. 3. Cyclin B is mislocalised in HeLa cells after geldanamycin treatment. (A-F) Immunostaining of HeLa cells either after incubation with DMSO (A,C,E) as a control or with geldanamycin (B,D,F). Cyclin B is shown in red (left panel) and centrosomes and MTs are visualised with anti-TACC3 antibodies in green (middle panel); DNA is shown in blue in the merged images (right panel). Before NEB, cyclin B can be detected at centrosomes in both control cells and in GA-treated cells (A and B, white arrows). In metaphase control cells, cyclin B decorates the mitotic spindle (C) whereas after GA incubation (D) cyclin B cannot be detected on the MTs. (E,F) Metaphase cells viewed from one end of the spindle. In control cells (E) cyclin B is seen on the centrosomes and MTs whereas after GA treatment (F) no clear centrosomal or MT staining is observed. (G) Bar graphs showing the quantification of cyclin B staining on centrosomes or on MTs in control (green bars) and GA-treated cells (red bars). Bar, 10 µm (A-F).





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