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Figure 4


Fig. 4. Msps/ch-TOG is not required for the centrosomal or MT localisation of cyclin B. (A,B) Immunostaining of Drosophila neuroblasts cells showing cyclin B (red, left panel), tubulin (green, middle panel) and DNA (blue in merged panel). (A) msps mutant neuroblast cell in prophase. Although the poles are somewhat disorganised in this cell, cyclin B is associated with the centrosomes. (B) msps mutant neuroblast cell in metaphase. Cyclin B is present at the poles and is associated with the mitotic spindle. (C,D) Bar graphs showing the quantification of cyclin B staining on centrosomes or on MTs in WT (green bars) and msps mutant (red bars) neuroblasts in prophase and prometaphase + metaphase. Bar, 5 µm (A,B). (E,F) Immunostaining of control and ch-TOG-depleted HeLa cells with cyclin B (red, left panel) and ch-TOG (green, middle panel); DNA is shown in blue in the merged panel. (E) In mock-depleted HeLa cells, both cyclin B and ch-TOG can be detected on centrosomes and MTs. Note that cyclin B has already disappeared from the centrosome in this cell that has completely aligned its chromosomes at the metaphase plate (Clute and Pines, 1999). (F) In ch-TOG partially depleted cells cyclin B associates with the spindle. Bar, 10 µm (C,D).





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