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Figure 5


Fig. 5. Hsp90 activity is required for Msps/ch-TOG localisation to spindle MTs. (A,B) Immunostaining of Drosophila third instar larvae neuroblasts with Msps (red, left panels), and {alpha}-tubulin (green, middle panels); DNA is shown in blue in the merged panels. (A) In WT metaphase cells Msps stains the centrosomes and MTs. (B) In Hsp90 mutant cells Msps can be detected on the centrosomes but not on the spindle MTs. (C,D) Immunostaining of HeLa cells either after incubation with DMSO (C) or with geldanamycin (D). In control cells, ch-TOG (red, left panels) strongly decorates the MTs (green, middle panels) of the mitotic spindles but in GA-treated cells there is a decrease in MT staining. (F) Bar graphs showing the quantification of Msps staining in WT (green bars) and Hsp90 mutant (red bars) Drosophila neuroblasts in prometaphase and metaphase. (G) Bar graphs showing the quantification of CH-TOG staining in control (green bars) and GA-treated (red bars) HeLa cells in prometaphase and metaphase. Bars, 5 µm (A,B); 10 µm (C,D).





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