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Figure 4


Fig. 4. Localization of cis-Golgi markers in the tubular networks. Cells from cricket fat bodies were infected with P. grylli and treated with an aqueous 1% solution of OsO4 for 24 hours (A) or prepared for cryo-immuno EM (B-H). (A) Arrow shows TN stained with osmium black. (B) Immuno-gold EM staining of cryosections for GNA lectin in the sporoblast. (C,D) Immuno-EM localization of {gamma}COP in the meront (C) and sporont (D). (E,F) Immunolocalization of Sec13 in meronts (E,F, arrow) and in the sporont (F, right side). The plasmalemma of the sporont is indicated with a dashed line. (G,H) Immunolocalization of giantin-like (G, thick arrow over round profiles) and GM130-like (H, thick arrow) proteins in meronts; thin arrows in G indicate microsporidia plasma membrane. Bar, 250 nm (A); 100 nm (B-F); 180 nm (G); 120 nm (H).





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