spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 5


Fig. 5. Colocalization of wt and mutant Nod2 proteins with endogenous Rac1. (A) Rac1 colocalization with F-actin in membrane ruffles. COS-7 cells were stained for Rac1 with the anti-Rac1 mAb and for F-actin with TRITC-phalloidin. Images were obtained by confocal microscopy (Leica TCS NT). (B) COS-7 cells were transfected with the following amounts of plasmid encoding the HA-tagged or FLAG-tagged wt or mutant Nod2 proteins: pcDNA3-HA-wt Nod2 and pcDNA3-HA-{Delta}CARDs (1 µg each), pcDNA3-HA-{Delta}LRRs (4 µg), pcDNA3-HA-CARDs (0.5 µg), pcDNA3-HA-LRRs (2 µg), pcDNA3-FLAG-NOD (2 µg). In each case, the total DNA amount was adjusted to 4 µg with pcDNA3. Twenty-four hours post transfection, cells were stained for the wt or mutant Nod2 proteins with monoclonal anti-HA or rabbit anti-FLAG antibodies and for endogenous Rac1 with a monoclonal antibody. Images were obtained by confocal microscopy (Leica TCS NT). Arrows indicate areas of colocalization.





Right arrow Return to article