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Figure 10


Fig. 10. (A) Western blot immunostained for pSMAD2, pMAPK14, pMAPK3/1 and ACTB in COCs treated with EGF (10 ng/ml) alone or together with SB431542 (10 µM) for 4 hours. Graphical representations of pSMAD2, MAPK14 and MAPK3/1 pixel intensity normalized to ACTB are shown below a representative western blot (n=3). (B) Western blot immunostained for pSMAD2, total SMAD2 and ACTB in COCs treated with EGF (10 ng/ml) for 0, 4, 8 and 12 hours. Graphical representations of pSMAD2, total SMAD2, MAPK14 and MAPK3/1 pixel intensity, normalized to ACTB, are shown below a representative western blot (n=4). Note that each image shown is of the same blot sequentially stained for the proteins indicated. abcP<0.05, significant differences.





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