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Figure 1


Fig. 1. BrdU incorporation increases after a delay in proliferating hippocampal NPCs following a single 24-hour pulse (but not repetitive pulses) of NMDA. (A) Diagram illustrating experimental procedures. (B) Micrographs of cells comparing of BrdU incorporation following treatment with a single pulse of NMDA (5 µM) over 1 day (NMDA 1'), two pulses over 2 days (NMDA 2') or three pulses over 3 days (NMDA 3') in the presence of bFGF. Cells were fixed on 9 DIV. Bar, 100 µm. (C) Graph showing BrdU incorporation in cells treated as in B; n=8. (D) BrdU incorporation following single pulses for 24 hours. Proliferating cells were treated with NMDA (5 µM), MK801 (5 µM), NaCl (5 mM), KCl (5 mM), AMPA (5 µM), or CNQX (10 µM). The percentage of BrdU+ cells [BrdU (+)] is shown. Insets, All the cells were counterstained with DAPI (blue), and total and BrdU+ cells were counted with a microscope in ten non-overlapping fields per well. Each value represents the mean ± s.e.m. of eight (B,C) and four (D) independent experiments. CTL, control; *P<0.05,**P<0.01, ***P<0.001.





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