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Figure 7


Fig. 7. NMDA receptor subunit gene expression during NMDA-induced proliferation and differentiation. Gene expression was investigated by semi-quantitative RT-PCR (A) and quantitative RT-PCR (B). Proliferating NPCs were treated with NMDA for 24 hours in the presence of bFGF and mRNA was extracted 72 hours after the stimulation. For differentiation, NPCs were treated daily with NMDA for 3 days in NB medium, and mRNA was isolated 24 hours after the last stimulation. The primer sets used for amplification of different subunits are listed in Materials and Methods. *P<0.05, **P<0.01, ***P<0.001.





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