JCS000018 Supplementary Material

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• Supplemental Figure 1 -

  Fig. S1. OVCAR-3 cells were treated with 10 nM OA or transiently transfected with HREV1FL, dNdC and pcDNA3. Forty-eight hours after treatment or transfection, subcellular fractions were purified and the distribution of the H-REV107-1, PR65 and PR36 proteins within these fractions was analyzed by western blotting using corresponding specific antibodies. Purity of the cytoplasmic and nuclear fractions was controled by histone 3 and α-tubulin. Each experiment was performed at least twice, the observed moderate differences in the amount of proteins in different fractions after transfection with H-REV107-1 wild type and its mutated form could not be reproduced in every experiment and therefore were concluded to be irrelevant for the inhibiting effect of H-REV107-1 on the PP2A catalytic activity and was not further analyzed.