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Figure 2


Fig. 2. Onco intracellular pathway. HeLa cells were incubated for 45 minutes with Onco-Red and Tf-Cy5 (to label early endosomes). When indicated, cells were also labeled with dextran-FITC, a tracer that is addressed to lysosomes. Cells were then processed for immunodetection of markers for late endosome/lysosomes [Lamp-1, Lamp-2 or lysobisphosphatidic acid (LBPA)], the ER (calnexin) or the trans-Golgi network (TGN46), before mounting and observation under a confocal microscope. Median optical sections; bar, 10 µm. Boxed areas in LBPA and Lamp-2 images indicate regions enlarged on the right.





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