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Fig. 3. Colocalization of Abi1, Bcr-Abl and WAVE2 with an abnormal F-actin-rich structure. Ba/F3 cells expressing p185^wt were probed with anti-Abi1 (a-c) and anti-Abl (d-f) antibodies, respectively. This was followed by staining with FITC-conjugated secondary antibody. Cells were then counterstained with TRITC-conjugated phalloidin and DAPI to visualize F-actin and nuclei, respectively. In g-i, the Ba/F3 cells expressing p185^wt were transduced with retrovirus expressing GFP-WAVE2 and counterstained by TRITC-conjugated phalloidin and DAPI. Subcellular distribution of Abi1 (a, green), Bcr-Abl (d, green), GFP-WAVE2 (g, green) and F-actin structures (b,e,h, red) were visualized by two-photon confocal microscopy, as indicated by arrowheads. The colocalization of these proteins with abnormal F-actin structure is shown by merged images (c,f,i). Bar, 10 µm.

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