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Fig. 4. The expression of p185^Bcr-Abl induced integrin clustering. (A) 1-integrin clustering in p185^wt-transformed Ba/F3 cells. The Ba/F3 cells transduced with indicated retroviruses were starved, fixed and stained with FITC-conjugated monoclonal antibody against 1-integrin. Cells were then stained with DAPI to visualize nuclei. Images were captured by two-photon confocal microscopy and the 1-integrin clustering is indicated by arrowheads. Bar, 10 µm. (B) Colocalization of 1-integrin, paxillin and vinculin with abnormal actin-enriched structure. The Ba/F3 cells expressing p185^wt were starved, fixed, permeabilized and probed with FITC-conjugated antibodies against 1-integrin, paxillin, tubulin and vinculin (green), as indicated. The cells were then counterstained with TRITC-conjugated phalloidin (red) and DAPI (blue) to visualize F-actin and nuclei, respectively. Images were captured by two-photon confocal microscopy and the colocalization of integrin, paxillin and vinculin with abnormal actin-enriched structure is shown in merged images, as indicated by arrowheads. Bar, 10 µm.

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