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Figure 2


Fig. 2. Extension of stabilized MTs in PC dendrites is inversely correlated with the stathmin mRNA level. (A) Immunohistochemistry of MAP2 and acetylated {alpha}-tubulin. Brains were dissected at P9, P12, P15, or P18. Lobule 4-5 (L4-5) is shown. Abbreviations are the same as in Fig. 1. Bar, 50 µm. (B) `Signal length' of MAP2 and acetylated (Ac) {alpha}-tubulin along the PC dendrites was quantified per area of the molecular layer of L4-5 using a total of 18 images from three animals for each time point. Images used for quantification were two views from three serial and sagittal sections (10 µm thick each) per an animal. Purkinje cells flatly extend the dendrites along the sagittal plane, and it is predicted that the whole dendrites of each Purkinje cells are included in the three serial sections. The signal intensity of stathmin mRNA in PC somata is the same as that shown in Fig. 1E. Error bars indicate mean ± s.d.





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