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Figure 6


Fig. 6. Overexpression of wild-type SCG10 or an S50A mutant similarly reduced dendritic growth of cultured PCs. (A) Subcellular distribution of stathmin and SCG10 in cultured PCs at DIV-9. (B) High-magnification micrographs of SCG10- or S50A-overexpressing PCs in primary culture. The micrographs are merged images of calbindin (red) and Myc (green) signals. Both endogenously and ectopically expressed SCG10 localize mainly in the perinuclear region (arrows). (C) Immunocytochemistry of GFP-, SCG10-, or S50A-overexpressing PCs in culture at DIV-14 as described in Fig. 3B. Arrowheads demarcate the axons of transfectants. (D) Quantification of total dendritic branch length of GFP-, SCG10-, and S50A-overexpressing PCs in primary culture. Number of cells measured: GFP, n=26; SCG10, n=20; S50A, n=23. Data are presented as mean ± s.e.m. Bars, 20 µm (A,B), 50 µm (C).





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