|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
|
Fig. 6. Syn5 and Syn16 RNAi effects on cell intoxication by STx, CTx and ricin. (A) Mock-transfected control cells or HeLa cells transfected with the indicated siRNAs were treated for 1 hour at 37°C with the indicated doses of STx. Protein biosynthesis was then determined by measuring the incorporation of radiolabeled methionine into acid-precipitable material. Data are presented as percentage of protein biosynthesis under the indicated conditions when compared with non-toxin-treated cells. (B) Cells were treated for 1 hour with the indicated doses of STx and analyzed as described in A. (C) Mock-transfected or RNAi-transfetced cells were treated with increasing doses of CTx, and cAMP production was measured to detect CTx arrival in the cytosol. (D) Mock-transfected or RNAi-transfetced cells were treated for 1 hour with the indicated doses of ricin, and analyzed as described in A. (E) Intoxication of HeLa cells by un-nicked (STx) or pre-nicked STx (Nicked) in mock-transfected control cells or Syn5 RNAi cells (siSyn5). Pre-nicking did not alter the Syn5-RNAi-mediated protection of cells against the toxin. (A-E) Means of two to four experiments.
|
