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Figure 2


Fig. 2. Altered level or activity of HDAC6 alters focal adhesion area. (A) Paxillin immunostaining of typical TC-7 cells, following 2-hour treatments as indicated. Bar, 20 µm. (B) Quantification of adhesion area in TC-7 cells (shown in A) treated with TSA, taxol and Nz (nocodazole), and NaB; see Materials and Methods for details of measurements of paxillin immunofluorescence. (C,D) Time course of the increase in (C) total focal adhesion area, and (D) average area of each individual focal adhesion. (E) Quantification of total focal adhesion area in NIH-3T3 cells stably expressing neomycin plasmid alone (NEO) or WT HDAC6. (F) Quantification of total focal adhesion area in WT, HDAC6 KO and KO MEFs transiently transfected with HDAC6 (HDAC6 rescue MEFs). (G) Quantification of effects of tubacin on total focal adhesion area in TC-7 cells. *, conditions statistically different from controls (P<0.05); ** in B, statistically significant difference both from control and from cells treated with TSA, taxol and Nz. ** in F, statistically significant difference both from WT control and from HDAC6 KO MEFs.





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