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Figure 2


Fig. 2. Actin concentration analysis. (a) Distribution of F-actin in keratocyte visualized with Rhodamine-phalloidin. Actin-concentration profile from front to back of a lamellipodium (boxed gray region) is plotted at the right panel. Black dashed box represents a typical (7x7 µm2) field of interest. Bar, 10 µm. (b) Contrast of the simulated images (circles) is plotted versus the inverse square root of actin-polymer concentration. Error bars give ± s.d. Gray area shows the range of contrast measured in experimental images and the corresponding range of actin concentration. (c,d) Histogram of the intensity ratio between front and back of a lamellipodium using measurements based on phalloidin-staining intensity (c), showing the same decay as the concentration profile measured by experimental contrast (d). Error bars give ± s.d. (e) Comparison of relative concentration profiles measuredon the basis of intensity (solid lines), contrast (dashed lines), in experimental (squares) and simulated images with zero-order and first-order depolymerization kinetics (circles and diamonds, respectively).





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