QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

First published online March 30, 2007

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Related articles in JCS Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Search for Related Content Social Bookmarking                         What's this?

LIMbering up to make muscle

Throughout life, myoblast differentiation maintains and repairs muscle. The transcription factor MyoD, which is regulated by MyoD-interacting proteins, initiates myogenesis by controlling the temporal expression of myogenin and other muscle-specific regulatory genes. Now, Christina Mitchell and colleagues identify FHL3 as a MyoD-interacting protein that negatively regulates myogenesis (see p. 1423). FHL3, which is highly expressed in skeletal muscle, contains four and a half LIM domains, which mediate protein-protein interactions. The authors show that overexpression of FHL3 in the muscle cell line C2C12 retards myotube formation and decreases the expression of myogenin but not MyoD. By contrast, knocking down FHL3 by RNAi enhances myoblast differentiation and increases myogenin expression, again without altering MyoD expression. FHL3, the authors report, binds to MyoD and functions as its potent negative co-transcriptional regulator. They propose, therefore, that, when new muscle is needed, FHL3 is excluded from the nucleus, which relieves its negative regulation of MyoD and allows myogenesis to proceed.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

Related articles in JCS:

FHL3 binds MyoD and negatively regulates myotube formation

Denny L. Cottle, Meagan J. McGrath, Belinda S. Cowling, Imogen D. Coghill, Susan Brown, and Christina A. Mitchell
JCS 2007 120: 1423-1435. [Abstract] [Full Text]  

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Related articles in JCS Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Search for Related Content Social Bookmarking                         What's this?