JCS001016 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. RT-PCR analysis of sca-1 from wild-type worms, wild-type worms grown on EGTA, csq-1(jh109) worms, and csq-1(jh109) worms grown on EGTA. Results a significant reduction of sca-1 transcription levels in csq-1(jh109) mutants. However, the transcription levels of sca-1 in csq-1(jh109) were increased by EGTA treatment. EF-1a was used as an internal control.

• Supplemental Figure 2 -

  Fig. S2. Cellular localization of calsequestrin mutants. (A) Transgenic line expressing the CSQ-1(K111A) mutant form stained with anti-CSQ-1 antibody shows diffused pattern. (B) The unc-68(e540) mutant stained with anti-CSQ-1 antibody show a dispersed pattern. (C, D) Transgenic lines transformed with construct K111Q show diffused patterns similar to that shown in A. (E) The transgenic line E247Q mostly show a diffused pattern similar to that shown in A, but infrequently show a weak dispersed pattern (F). (G) The transgenic line E251Q mostly show diffused pattern like (A), but infrequently show a weak dispersed pattern (H). (I, J) Transgenic line expressing the double mutant E247Q⁻E251Q⁻ show completely diffused pattern similar to that shown in A.

• Supplemental Figure 3 -

  Fig. S3. Functional rescue with rabbit calsequestrin in the csq-1-null mutant csq-1(jh109). Survival rates of wild-type and transgenic animals expressing rabbit skeletal calsequestrin under low Ca²⁺ conditions. Survival rate of worms with rescued CSQ-1 function was compared with that of N2-strain worms at 20°C. Over 50 animals were tested for each data point of a single set of experiments, and each experiment was repeated three times.