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Fig. 8. Cdk5 is required for virulence. (A) Maize plants were infected with wild-type control strains and cdk5^ts mutants and incubated at 22°C and 30°C. The graph shows the quantification (mean ± s.e.m.) of tumor formation on infected maize plants at 22°C and 30°C after 14 days. (B) Mating assays of control strains FB1 x FB2, and cdk5^ts mutant strains FB1 cdk5^ts x FB2 cdk5^ts on charcoal-containing agar plates at 22°C and 30°C. (C) Formation of conjugation hyphae after Fuz7DD overexpression in cells carrying a wild-type cdk5 allele (Pcrg:fuz7DD) and a cdk5^ts allele at 30°C. The image on the right shows a cdk5^ts cell doublet separated by a septum (arrow). Bar, 20 µm. (D) Formation of infective hyphae after bE/bW overexpression in cells carrying a wild-type cdk5 allele (AB31) and cdk5^ts allele at 30°C. Bars, 20 µm.

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