spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 3


Fig. 3. Accumulation of UBB+1 is reversible after shutting off expression. Flow cytometric analysis of the percentage of UBB+1 (A) or UBB+1/GFP (B) positive cells at 0 hours, 12 hours and 36 hours after shutting down maximal UBB+1 expression. UbG76V-GFP HeLa cells were not transfected (control), transfected with empty pcDNA3 vector (e) or transfected with the UBB+1 Tet-off vectors. After 64 hours of maximal expression (absence of Dox) a baseline sample was taken (0 hours). UBB+1 expression was continued at maximal levels (Dox–) or shut down by addition of 10 ng/ml Dox (Dox+) and samples were analyzed after 12 hours and 36 hours of Dox treatment. Results are the means ± s.e.m. of two or three independent duplicate experiments, *P<0.01; #p=0.099 ANOVA between groups indicated at 36 hours.





Right arrow Return to article