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Fig. 5. UBB⁺¹ is degraded by the proteasome in cortex slice cultures. Organotypic cortex slices of C57Bl/6 mice were transduced with LV-Ub^G76V-GFP, LV-UBB⁺¹ or LV-UBB^+1K29,48R. Both the UPS reporter protein Ub^G76V-GFP (green) and UBB⁺¹ (red) are efficiently degraded by the 26S proteasome and only accumulate after treatment with proteasome inhibitor. The lysine mutant of UBB⁺¹, UBB^+1K29,48R, is not degraded by the proteasome and accumulates without inhibitor treatment. – epox, not treated with epoxomicin; + epox, treated overnight with 1 µM epoxomicin. Bar, 100 µm.

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