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Fig. 6. UBB⁺¹ remains present after washout of inhibitor in cortex slice cultures. Overnight incubation of cortex cultures transduced with LV-Ub^G76V-GFP or LV-UBB⁺¹ with the reversible proteasome inhibitor MG132 (10 µM) resulted in accumulation of both proteins. Washing out the reversible inhibitor reactivated the proteasome, as shown by the degradation of the proteasome reporter substrate Ub^G76V-GFP. However, UBB⁺¹ remained in a considerable number of cells after reactivation of the proteasome. Transduction with the LV-UBB^+1K29,48R control construct gave rise to accumulation of the UBB⁺¹ protein regardless of proteasome inhibitor treatment. Ub^G76V-GFP is depicted in green, UBB⁺¹ in red, and the nuclear staining (TO-PRO) in blue. Bar, 500 µm.

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