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Figure 1


Fig. 1. The motile behavior of Dictyostelium moving on gelatin. (A-C) Stacked cell outlines, overlaid with a centroid track, from three representative cells of each type; wild-type, mlcE– and mhcA– cells. Examples of cells moving randomly at fast (1), intermediate (2) and slow (3) speeds, for each cell type, over a ~3-minute period are shown. (A'-C') Rose plots, showing the total distance traveled and the directionality of movement for each cell type during a ~3-minute period. Bar, 5 µm. (D-F) Summary histograms of the mean relative changes in instantaneous speed (D), persistence (E) and spread area (F) with respect to the wild-type value that was set to 100% for wild-type (black bar), mlcE+ (gray bar), mlcE– (striped bar) and mhcA– cells (open bar, n=9). Asterisks indicate statistically significant differences compared with wild-type cells (P<0.05), using a Student's t-test, assuming unequal variances.





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