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Fig. 4. Changes in distribution of traction stress generated by a wild-type Dictyostelium cell during a `slow recoil' retraction. (A-L) A time series of traction vector maps obtained from a wild-type cell moving in the general direction indicated (arrow). Traction stresses increase gradually (A-C), particularly at the rear (arrow in C), prior to retraction (R in C) and decrease during retraction (C-G). Protrusion (P in C) occurs simultaneously with retraction (C-G) at the front of cell where there are regions of low traction stresses. Bar, 3 µm. (M) Plots of the 90th percentile traction stress (red), instantaneous speed (green), cell area (blue) and shape factor (yellow) corresponding to panels A-L. A-G constitute one cycle of movement that includes the first slow recoil retraction (R) which occurs between 9 and 21 seconds, as indicated by the vertical dotted lines. H-L show the beginning of a second cycle of movement, prior to the onset of a slow recoil retraction (not shown). Note the gradual increase in traction stress at the rear and its similarity to panels A-D.
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