TGF3 inhibits E-cadherin gene expression in palate medial-edge epithelial cells through a Smad2-Smad4-LEF1 transcription complex

JCS003129 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Effect of antisense β-catenin ODN. Control immunoblotting demonstrated heavily decreased levels of β-catenin and γ-catenin in the presence of the antisense (AS) β-catenin ODN in fused MEE cells.

• Supplemental Figure S2 -

  Fig. S2. Quantitative assessment of chromatin immunopreciptiation PCR signals. (A-C) Real-time PCR showed results similar to those observed on agarose gels, with common association of Smad2-P, Smad4 and LEF1 to the LEF1 (non E-pal)-binding, LEF1 (E-pal)-binding and SBE-binding sites. These signals were increased upon exposure of cells to TGFβ3. Antibodies against IgG (negative control) and β-catenin showed no increase in ChIP signals.