spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 2


Fig. 2. Confirmation of Smad and LEF1 transcriptional activities. (A) p3TP-Lux reporter gene assay demonstrated that Smad transcriptional activity increased from unfused to fused MEE cells, with a sharp increase in activity upon stimulation with TGFbeta3. A dominant-negative Smad4 (DN Smad4) construct inhibited luciferase activity (mean ± s.d.; n=3; *P<0.05 compared with Unfused; **P<0.05 compared with p3TP-Lux). (B) pTOPFLASH-Lux (TF) reporter gene assay showed that LEF1 transcriptional activity continually increases as TGFbeta3 signaling progresses. Mutated LEF1 binding sites of the pFOPFLASH-Lux reporter, as well as treatment with DN Smad4 (Smad4 being necessary for LEF1 gene expression) or dominant-negative LEF1 (DN LEF1) inhibited transcriptional activity whereas antisense beta-catenin/{gamma}-catenin oligodeoxynucleotide (AS B-catenin ODN) did not (mean ± s.d.; n=3; *P<0.001 compared with Unfused; **P<0.01 compared with TF).





Right arrow Return to article