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Figure 3


Fig. 3. TGFbeta3 signaling promotes formation of a Smad2-P–Smad4–LEF1 transcription complex. (A) Co-immunoprecipitation (IP) of cell extracts from MEE cells was performed using antibodies against Smad2-P, Smad4 or LEF1. Immunoblot (IB) analysis showed that Smad4, Smad2-P and LEF1 are bound together in the extracts of fused MEE cells; increased levels were observed when cells were exposed to TGFbeta3. Smad3-P and beta-catenin were not found in this complex. Unfused MEE cells showed no traces of interaction because LEF1 is not expressed in these cells. (B) Immunoprecipitation and immunoblotting for beta-actin was used as an internal control.





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