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Figure 2


Fig. 2. ERK1/2 and Rho activation mediated by WT and AAA mutant P2Y2Rs. Human 1321N1 cells were transiently transfected with either WT (RGD) or AAA mutant P2Y2Rs in pcDNA3.1(–). Transfected cells were starved overnight and stimulated with the indicated concentration of UTP for 5 minutes prior to measuring ERK1/2 phosphorylation (A) or Rho activity (B). (A) UTP-induced with 1 mM UTP for 5 minutes before measuring ERK1/2 phosphorylation is expressed as fold increase over basal level in cells transfected with WT P2Y2R. Data points represent the mean ± s.e.m. of results from three experiments. (B) The Rho-GTP band density was normalized to total Rho and relative intensities are shown below each band as mean ± s.e.m. of results from four experiments.





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